Table 1 Bacterial strains used in the study.

From: Identification of Acinetobacter baumannii and its carbapenem-resistant gene blaOXA-23-like by multiple cross displacement amplification combined with lateral flow biosensor

Strain ID

Strains

Sources of strains (No. of strains)a

Duplex MCDA-LFB b

pgaD

blaOXA-23-like

1

Acinetobacter baumannii

SG-AB001

P

P

2–50

Acinetobacter baumannii

Isolated strain(49)

P

P

51–53

Acinetobacter baumannii

Isolated strains (3)

P

N

54

Listeria monocytogenes

Isolated strains (1)

N

N

55

Bacillus cereus

Isolated strain (1)

N

N

56

Citrobacter braakii

Isolated strain (1)

N

N

57

Citrobacter freundii

Isolated strain (1)

N

N

58

Corynebacterium ammoniagenes

Isolated strain (1)

N

N

59

Escherichia coli

ATCC35218

N

N

60

Klebsiella pneumoniae

ATCC2146

N

N

61

Proteus mirabilis

Isolated strain (1)

N

N

62

Providencia rettgeri

Isolated strain (1)

N

N

63

Pseudomonas aeruginosa

ATCC27853

N

N

64

Serratia marcescens

Isolated strain (1)

N

N

65

Serratia marcescens

Isolated strain (1)

N

N

66

Staphylococcus aureus

ATCC29213

N

N

67

Staphylococcus epidermidis

Isolated strain (1)

N

N

68

Streptococcus salivarius

Isolated strain (1)

N

N

  1. aATCC, American Type Culture Collection; SG, Shougang Hospital; AB, Acinetobacter baumannii; bP, positive; N, negative. Only these strains belonging to the A. baumannii could be detected by the MCDA-LFB technique, indicating the extremely high selectivity of the method. Particularly, pgaD and blaOXA-23-like genes can be simultaneously detected and correctly identified in a reaction vessel.
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