Figure 4 | Scientific Reports

Figure 4

From: Assessing the reliability of gene expression measurements in very-low-numbers of human monocyte-derived macrophages

Figure 4

Relative gene expression of human MDMs seeded in 6-well or 96-well plates, 24 h after stimulation with LPS and poly(I:C) (M1), IL4 and IL-10 (M2) or absence of stimulation (Mock). (A) qPCR analysis of 6-well samples. (B) qPCR analysis of 96-well samples. M1 and M2 values are relative to mock which is normalized to 1. Average RQs of three technical replicates ± SD are shown. Technical replicates: individual wells of a 6-well plate, pool of two wells of a 96-well plate. Reference genes: SDHA, HPRT1. (C,D) Scatter plots showing the statistical comparison between seeding conditions for matched treatments (C = M1, D = M2). Dashed lines correspond to the significance level ɑ = 0.05.

Back to article page