Figure 5 | Scientific Reports

Figure 5

From: Assessing the reliability of gene expression measurements in very-low-numbers of human monocyte-derived macrophages

Figure 5

Relative gene expression of human MDMs seeded in 6-well and 96-well plates, 24 h after M(LPS/IC) stimulation (M1), M(IL-4/IL-10) stimulation (M2), or absence of stimulation (Mock). (A) Average RQ values of 6-well samples. (B) Average RQ values of 96-well samples. M1 and M2 values are relative to mock which is normalized to 1. Bars represent means of monoplicates of 3 independent experiments ± SEM. Technical replicates: individual wells of a 6-well plate, pool of thirty-two wells of a 96-well plate. Reference genes: SDHA, 18S, GAPDH. (C) Scatter plot showing the statistical comparison between seeding conditions for matched treatments in M(LPS/IC) and M(IL-4/IL-10) stimulated macrophages. Dashed lines correspond to the significance level ɑ = 0.05.

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