Table 1 Seeding conditions of monocyte-derived macrophages in vessels of different size.

From: Assessing the reliability of gene expression measurements in very-low-numbers of human monocyte-derived macrophages

Multiple well plate

Area/well (cm2)

Seeded cell number/well

µl medium/well

6 well

8,87

100,000

4,000

12 well

3,90

44,000

1,760

24 well

1,90

21,400

860

48 well

1,00

11,300

450

96 well

0,32

3,610

140

  1. To keep the cell density identical, the cell number per vessel was calculated by dividing the vessel area by the reference vessel area (8,87 cm2) and multiplying the result by the standard cell number (100,000).
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