Figure 2
From: Abnormal development of zebrafish after knockout and knockdown of ribosomal protein L10a
(a) Pattern of heteroduplexes and homoduplexes observed after crRNA-tracrRNA-Cas9 complex (CRISPR-Cas9) injection. (b) HMA showing different heteroduplex and homoduplex patterns in the 5-bp deletion mutant compared to the wild-type (WT). (c) Schematics of the mutations obtained from genome editing. The crRNA target site and PAM sequences are presented with gray highlighting and red letters, respectively. Deleted nucleotides are shown with hyphens, and inserted nucleotides are in blue letters. (d) Predicted amino acid sequences after rpl10a gene knockout by CRISPR-Cas9 starting from amino acid position 190. The deleted sequences and stop codons are marked with hyphens and stars, respectively.
