Table 3 Half-maximal inhibitory concentrations (IC₅₀) and fluorescence quenching parameters of major compounds and flavonol aglycones.

Compound	IC₅₀ (μM)	Temperature (°C)	K_SV (10⁴ M⁻¹)*	k_q (10¹² M⁻¹s⁻¹)^**	K_A (10⁴ M⁻¹)^***	n^****	R¹	R²	R³
Quercetin	53.05	25	1.00	6.29	—	—	OH	H	H
Quercetin	53.05	37	0.94	5.91	3.57	1.1471	OH	H	H
Kaempferol	79.38	25	1.22	7.67	—	—	H	H	H
Kaempferol	79.38	37	1.03	6.48	2.07	1.1103	H	H	H
Myricitrin	92.85	25	1.19	7.48	—	—	OH	OH	α-L-rhamnopyranosyl
Myricitrin	92.85	37	1.23	7.74	1.45	1.0228	OH	OH	α-L-rhamnopyranosyl
Quercitrin	100.56	25	1.28	8.05	—	—	OH	H	α-L-rhamnopyranosyl
Quercitrin	100.56	37	1.32	8.30	1.00	0.9760	OH	H	α-L-rhamnopyranosyl
Avicularin	141.84	25	0.56	3.52	—	—	OH	H	α-L-arabinofuranosyl
Avicularin	141.84	37	0.59	3.71	0.94	1.1119	OH	H	α-L-arabinofuranosyl

R¹, R², and R³ are substituent positions on the basic structure of flavonol in Fig. 2.
^*Stern-Volmer quenching constant; ^**Bimolecular quenching constant; ^***Binding constant; ^****Number of binding sites.

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