Figure 3
From: Participation of 5-lipoxygenase and LTB4 in liver regeneration after partial hepatectomy
mRNA and protein expression of main enzymes for LT synthesis in rat liver 1, 5 and 24 h post-PH in animals treated with vehicle or zileuton. RT-qPCR of (a) 5-LOX, (c) LTA4-H and (e) LTC4-S. Immunoblotting of (b) 5-LOX, (d) LTA4-H and (f) LTC4-S. Selected lanes were cropped from different gels and they are shown after cropping, aligning and separating them by white space. Full-length blots are available in Supplementary Information. The amount of every protein band was corrected by β-actin but not shown in order to avoid a more complex image. Sh: sham animals, PH: partial-hepatectomized animals treated with vehicle, PHZi: PH-animals treated with zileuton 40 mg/Kg body weight. Bars represent mean ± SEM (n = 4 per experimental group). *p < 0.05 vs. Sh, #p < 0.05 vs. PH for each time.
