Figure 4
From: Participation of 5-lipoxygenase and LTB4 in liver regeneration after partial hepatectomy
Analysis of zileuton effect on NF-κB activation 1 h post-PH. (a) NF-κB binding activity measured with a commercial kit. (b) Immunoblotting of the NF-κB inhibitor, IκB-α, in total lysates. (c) Analysis of mRNA expression by RT-qPCR of IL-6. Selected lanes were cropped from different parts of the same gel and they are shown after cropping, aligning and separating them by white space. Full-length blots are available in Supplementary Information. Sh: sham animals, PH: partial-hepatectomized animals treated with vehicle, PHZi: PH-animals treated with zileuton 40 mg/Kg body weight. Bars represent mean ± SEM (n = 4 per experimental group). *p < 0.05 vs. Sh, #p < 0.05 vs. PH.
