Figure 5
From: Participation of 5-lipoxygenase and LTB4 in liver regeneration after partial hepatectomy
Effect of zileuton treatment on neutrophils recruitment after partial hepatectomy. (a) Mieloperoxydase (MPO)-positive cells quantification in liver tissue from Sh, PH and PHZi rats at 1 and 5 h post-PH. (b) Representative images of MPO immunostaining 5 h post-PH obtained by optical microscopy (400X). Analysis of mRNA expression of (c) MPO and (d) elastase in isolated liver leukocytes 5 h after PH. (d) Quantification of recruited liver neutrophils 5 h post-PH assessed by immunofluorescence and confocal microscopy. (e) Representative images from immunofluorescence detection of neutrophils (100X magnification). Sh: sham animals, PH: partial-hepatectomized animals treated with vehicle, PHZi: PH-animals treated with zileuton 40 mg/Kg body weight. Bars represent mean ± SEM (n = 4 per experimental group). *p < 0.05 vs. Sh, #p < 0.05 vs. PH.
