Figure 3

Elastica van Gieson (EVG) and Picrosirius Red (PSR) staining of the AAA wall in experiment 1 (A–D) Representative images of EVG staining (scale bar = 100 µm). (E) Elastin degradation scores in the control ligation and OVX ligation groups. (F–K) Representative images of PSR staining (scale bar = 50 µm). (L) Quantification of the collagen-positive area of the vascular wall in the control ligation and OVX ligation groups. Data are represented as mean ± S.E.M. (E,L) Control ligation group (n = 13), OVX ligation group (n = 9). Values with different letters are significantly different (P < 0.05). The arrows show the direction of intima and adventitia. In the case of enlarged figure, all layers of arterial wall were not shown.