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Figure 2

From: Structural and biochemical basis of the formation of isoaspartate in the complementarity-determining region of antibody 64M-5 Fab

Figure 2

Charge heterogeneity and time-dependent change of 64M-5 Fab with cation-exchange chromatography. (A) An elution profile on a Mono S cation-exchange column. The solid line indicates absorbance at 280 nm of eluates, and the broken line indicates the ionic concentration. The Fr. 1 isoform was used for subsequent crystallographic analyses. (B) Elution profiles on a Mono S cation-exchange column after incubating the Fr. 2 isoform of 64M-5 Fab at pH 7.5 and 37 °C.

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