Figure 2
From: Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria
Signals for PI (X axes) and 6-FAM (Y axes) are plotted to show the sorting of (i) Pseudovibrio sp. SB55 when hybridized with (A) the probe PARA739_6-FAM, (B) the “non-target” LGC339_6-FAM probe, (C) “no probe” and (D) PARA739_6-FAM + PI; (ii) Bacillus sp. AU29 when hybridized with (A) the probe LGC339_6-FAM, (B) the “non-target” PARA739_6-Fam probe, (C) “no probe” and (D) LGC339_6-FAM + PI; (iii) the mock community when hybridized with (A) the probe PARA739_6-FAM for Pseudovibrio sp. SB55, (B) the “non-sense” NONEUB338_6-FAM probe, (C) “no probe” and (D) PARA739_6-FAM + PI; (iv) Pseudovibrio sp. SB55 inoculated into Baltic seawater when hybridized with (A) the probe PARA739_6-FAM, (B) the “non-sense” NONEUB338_6-FAM probe, (C) “no probe” and (D) PARA739_6-FAM + PI. In all D panels, either the gates or the quadrants indicate the cell populations that were distinguished after counterstaining with PI and sorted.
