Figure 3
From: Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria
Signals for PI (X axes) and 6-FAM (Y axes) are plotted to show the sorting of Alphaproteobacteria from the Baltic seawater bacterial community after hybridization with the probe ALF968_6-FAM. (A) The arrows indicate the two populations of the unstained seawater bacterial community showing green and red autofluorescence. (B) The arrow points at the 6-FAM positive population after hybridization with the probe ALF968_6-FAM. (C) Negative control using the “non-sense” NONEUB338_6-FAM probe. (D) Negative control using “no probe” at all. (E) After counterstaining with PI, two populations were gated and sorted: 6-FAM positive and PI negative, and 6-FAM positive and PI positive.
