Table 3 CFU ml−1 values at all steps of the modified FFF protocol and percentage viability (in brackets) with respect to values after overnight incubation (control) for both Bacillus sp.

From: Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria

FISH steps

Bacillus sp. AU29 CFU ml−1(%)

Ruegeria sp. AU82 CFU ml−1(%)

Control

5.80E + 08

3.70E + 08

Pre-hybridization

7.20E + 07 (12.41)

5.20E + 07 (14.05)

Heat shock

3.07E + 07 (5.30)

1.07E + 07 (2.90)

Hybridization

8.00E + 06 (1.38)

9.80E + 06 (2.65)

Washing

5.73E + 05 (0.10)

2.73E + 05 (0.07)

  1. AU29 and Ruegeria sp. AU82.
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