Table 4 CFU ml−1 values at all steps of the modified FFF protocol and percentage viability (in brackets) with respect to values after overnight incubation (control) for Bacillus sp.

From: Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria

FISH steps

Bacillus sp. AU29 CFU ml−1(%)

Ruegeria sp. AU82 CFU ml−1(%)

Pseudovibrio sp. SB55 CFU ml−1(%)

Control

7.20E + 08

5.31E + 08

6.50E + 08

Pre-hybridization

4.90E + 08 (68.06)

3.82E + 08 (71.93)

2.89E + 08 (44.46)

Heat shock

2.09E + 08 (29.05)

1.59E + 08 (29.88)

1.02E + 08 (15.69)

Hybridization

7.44E + 07 (10.34)

2.20E + 07 (4.14)

1.34E + 07 (2.06)

Washing

8.90E + 06 (1.24)

1.50E + 07 (2.82)

1.01E + 07 (1.55)

  1. AU29, Ruegeria sp. AU82 and Pseudovibrio sp. SB55.
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