Figure 3 | Scientific Reports

Figure 3

From: Glycogen Synthase Kinase-3β Inhibition with 9-ING-41 Attenuates the Progression of Pulmonary Fibrosis

Figure 3

IPF fibroblasts demonstrate increased GSK-3β nuclear localization. Normal and IPF fibroblasts were seeded on glass coverslips. Serum-starved cells were then treated with TGF-β for 48 h. Cells were then fixed, permeabilized and immunostained for GSK-3β. GSK-3β (green) and nuclei (red) were then visualized by confocal microscopy. Images are representative of 10 fields/slide and n = 3 samples/condition. Images were taken at 40X optical zoom. The Mean Fluorescence Intensity (MFI) of nuclear GSK-3β was graphed as mean ± SEM. *Denotes p < 0.05, **p < 0.01. Bar indicates 25 um.

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