Figure 2 | Scientific Reports

Figure 2

From: Functional importance of NUDT9H domain and N-terminal ADPR-binding pocket in two species variants of vertebrate TRPM2 channels

Figure 2

Typical kinetics of ADPR-dependent currents of drTRPM2, nvTRPM2 and hTRPM2 as obtained from inside-out patches. (a–c) Representative current traces of HEK-293 cells expressing the respective TRPM2 orthologue as indicated. Stimulation was performed with a bath solution containing Ca2+ (1 µM) and ADPR (as indicated). In the presence of ADPR the currents of drTRPM2 and nvTRPM2 developed almost instantaneously and quickly returned to baseline after ADPR removal. In contrast, in hTRPM2 the time course of both on- and off-kinetics is markedly delayed during application and withdrawal of ADPR (note the different time scales). Values are given in Table 1. (d) Single channel current amplitudes at indicated voltages were used to determine the conductance of drTRPM2, nvTRPM2 and hTRPM2 (e). Conductance significantly and gradually increases from hTRPM2 to drTRPM2 and nvTRPM2. All data are presented as mean ± s.d. differences are significant at ***(P < 0.001) and ****(P < 0.0001) evaluated with a one-way ANOVA and the Bonferroni correction, n = 3.

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