Table 4 Ligand-observed NMR analysis to assess compound 9 binding to wild-type and mutant variants of TNKS2 ARC4.
TNKS2 ARC4 constructs | T2 relaxation-edited | waterLOGSY |
|---|---|---|
compound alone | — | no NOE |
wild-type | 42.4% reduction | negative NOE |
arginine cradle (WFE591/593/598AAA) | 43.8% reduction | negative NOE |
central patch (L560W) | 22.4% reduction | positive NOE |
aromatic glycine sandwich (YY536/569AA) | 0% | positive NOE |
