Figure 1 | Scientific Reports

Figure 1

From: 3β, 6β-dichloro-5-hydroxy-5α-cholestane facilitates neuronal development through modulating TrkA signaling regulated proteins in primary hippocampal neuron

Figure 1

Optimization of the effective concentration of SCH for neurite outgrowth activity. Primary culture of embryonic hippocampal neurons was incubated with vehicle [DMSO, <0.5% (v/v)] or SCH at indicated concentrations for three days. Representative photomicrographs from the cultures incubated with different concentrations of SCH (a). Dio-stained images were inverted and presented as a grayscale mode with black neurite path and white background. Scale bar, 60 μm, applies to all images. Morphometric analysis for the number (b) and the total length of primary neurites, TLPN (c), and branching points (d) demonstrates that SCH promotes neurite outgrowth in a concentration-dependent manner. Statistical significance compared to vehicle: *p < 0.05, **p < 0.01 and ***p < 0.001 (ANOVA). Data points represent the mean ± SEM (n = 20 individual neurons). SEM, standard error of mean.

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