Figure 2 | Scientific Reports

Figure 2

From: 3β, 6β-dichloro-5-hydroxy-5α-cholestane facilitates neuronal development through modulating TrkA signaling regulated proteins in primary hippocampal neuron

Figure 2

Effects of SCH on the neuronal polarity. Neurons were grown on the same culture conditions as indicated in Fig. 1 for 24 hrs and 48 hrs, and then fixed and double-immunostained for MAP2 (green) and Tau (red), an axon and a dendrite specific markers, respectively. Fluorescent images showing early developmental stages (a); Lower panel: stage 1 (lamellipodia stage, indicated by arrow-heads), stage 2 (minor process stage, indicated by arrow-heads), stage 3 (axonal sprouting, indicated by arrow-heads). Scale bar, 80 μm, applies to all images of the upper panel. Percentage of neurons that reached different developmental stages at 24 hr (b) and 48 hr (c) of incubation. Statistical significance compared to vehicle: ***p < 0.001 (Student’s t-test). Data points represent the mean ± SEM (n = 3, each replicate with ~1000 neurons). SEM, standard error of the mean.

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