Figure 6 | Scientific Reports

Figure 6

From: 3β, 6β-dichloro-5-hydroxy-5α-cholestane facilitates neuronal development through modulating TrkA signaling regulated proteins in primary hippocampal neuron

Figure 6

Neurite outgrowth activity of SCH is TrkA-dependent. Hippocampal neurons were grown on the same culture conditions, as indicated in Fig. 1 in the presence of vehicle, GW441756 (TrkA inhibitor, 1 μM), SCH (30 μM) or SCH + GW441756 for two days. Representative phase-contrast photomicrographs from each treatment (a). Scale bar, 80 μm, applies to all images. Morphometric analysis for the number (b), and the total length of primary neurites (c), and longest neurite length (d) demonstrates that GW441756 caused a reduction in the activity of SCH on neurite extension. Statistical significance compared to vehicle: **p < 0.01 and ***p < 0.001 (ANOVA). Data points represent the mean ± SEM (n ≥ 20 individual neurons).

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