Figure 2 | Scientific Reports

Figure 2

From: Caveolin-2 deficiency induces a rapid anti-tumor immune response prior to regression of implanted murine lung carcinoma tumors

Figure 2

LLC tumor infiltrating CD4 and CD8 T cells as well as macrophages in WT versus Cav-2 KO mice analyzed by flow cytometry. Tumors were extracted at day 8 after s.c. implantation of LLC cells into flanks (106/flank) of WT and Cav-2 KO mice and single cell suspensions were prepared and stained with fluorescence-conjugated antibodies for flow cytometry as described in Methods. (A–C) Flow cytometry analysis of single cell suspensions obtained from LLC tumors and stained for detecting CD4 T cells (CD3+CD4+), CD8 T cells (CD3+CD8+) and macrophages (CD11b+F4/80+), respectively. Left and central panels: representative dot plots with indicated percentages of T cells and macrophage populations within total cells in LLC tumors from WT and Cav-2 KO mice, respectively. Right panels: percentages of T cells and macrophages calculated based on the respective dot plots. All plots were gated on total live cells. Data are from distinct samples and presented as the mean ± SEM. **p < 0.01, ***p < 0.001 compared with WT by unpaired t-test; n = 4.

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