Figure 3 | Scientific Reports

Figure 3

From: Caveolin-2 deficiency induces a rapid anti-tumor immune response prior to regression of implanted murine lung carcinoma tumors

Figure 3

mRNA and protein levels for selected surface markers and cytokines in LLC tumor lysates from WT versus Cav-2 KO mice. (A,B) RNA was extracted from tumors 5 days and 8 days after s.c. implantation of LLC cells into WT and Cav-2 KO mice and mRNA levels were determined by RT-qPCR. The values are presented as the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001 vs. WT determined by unpaired t-test; n = 4. (C) Protein lysates were generated from tumors extracted from WT and Cav-2 KO mice 5 and 8 days after s.c. injection of LLC cells. Concentrations of each cytokine were determined using multiplex bead-based Discovery assay by Eve Technologies and normalized per mg of protein. Data are from distinct samples and presented as the mean ± SEM. *p < 0.05, ***p < 0.001 compared with WT mice by unpaired t-test; n = 4.

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