Figure 5
The effect of bone marrow transfer from Cav-2 KO and WT mice on LLC tumor growth. WT and Cav-2 KO mice irradiated with a lethal dose of 1000 Rads using an X-RAD 320 (X-RAY) received Cav-2 KO and WT bone marrow transfer (Cav-2 KO BMT and WT BMT, respectively) via intravenous (tail vein) injection of 5 × 106 bone marrow cells isolated from murine femurs and tibia. 8 weeks after BMT, mice were s.c. injected with LLC cells (106) into two flanks and tumor growth in WT + Cav-2 KO BMT (A,C,E) and Cav-2 KO + WT BMT (B,D,F) were compared to non-irradiated WT and Cav-2 KO control mice, respectively. A and B: graphical representations of LLC tumor growth in WT + Cav-2 KO BMT vs. WT and in Cav-2 KO + WT BMT vs. Cav-2 KO mice, respectively. Tumor width and length were determined using a caliper, tumor volumes were calculated according to the formula: Volume = 0.52 × (width)2 × (length), and data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with WT (A) and Cav-2 KO (B) by two-way ANOVA followed by Bonferroni post-test; (C,D) Representative photographs of LLC tumors extracted on day 23 of the experiment. (E,F) Tumors extracted on day 23 (shown in C and D) were weighted and the average tumor mass ± SEM was calculated. Data are from distinct samples and presented as the mean ± SEM. *p < 0.05 and ***p < 0.001 compared with WT (E) and Cav-2 KO (F) by unpaired t-test (n = 12).
