Figure 5
WIP1 and HER2 co-inhibition in different cell lines. We measured cell viability (y-axis) of cell lines with (MCF7, BT474, MDA-MB-361 and ZR-75-30) or without (HCC1954 and SKBR3) PPM1D amplification. Some cell lines are TP53 wildtype (MCF7 and ZR-75-30), colored black; some harbor a TP53 missense mutation (HCC1954, SKBR3 and BT474), colored red and one harbors a TP53 nonsense mutation (MDA-MB-361), colored blue. Cell lines were treated with HER2 inhibitors and with GSK2830371, a WIP1 inhibitor. Cell viability decreased by WIP1 inhibition in some of the PPM1D amplified cell lines (pane A, B and D) but not in PPM1D normal cell lines (pane E and F) and a PPM1D amplified, TP53 nonsense mutated cell line (pane C). We measured WIP1 levels by immunoblotting, elevated WIP1 protein levels were found in cell lines with PPM1D amplification compared to cell lines without PPM1D amplification (pane G.).
