Figure 2
In vitro infectivity of L. infantum axenic amastigotes and promastigotes. (A) Images of peritoneal macrophages infected with either luciferase-expressing L. infantum axenic amastigotes (left) or promastigotes (right) at 4 hours or 72 hours post-infection and stained with Hemacolor. Asterisks indicate the magnified cell in the upper left corner of the image (B) Percentage of cells infected at 4 hours or 72 hours after infection. Data represented in bars corresponds to mean values + standard deviation represented in bars of experiment replicates. (C) Number of parasites per infected cell at 4 hours or 72 hours after infection. Each symbol represents the number of parasites per individual cell. Data from experiment replicates was pooled together. (B, C) AMA: infections were performed with axenic amastigotes. PRO: infections were performed with promastigotes. Statistical significance calculated by unpaired Student’s t test: (*)p < 0.05, (***)p < 0.001, (****)p < 0.0001. Data representative of two independent experiments.
