Figure 4
Isolation efficiency and capacity to remove protein contaminants of the ATPS-based EV isolation protocol. (a) Initial protein concentrations of EV containing biofluids. (b) Final concentrations of EVs isolated from different biofluids. (c) Protein concentrations of samples at different steps of the isolation process. (d) BCA measurement of 100 μg/mL BSA samples at various steps of isolation. (e) BCA measurement of various concentrations of BSA after two steps of washing. Protein standards were compared to their pre-isolation counterpart for significance (f) Pre-and-post isolation concentrations of EV samples with a range of starting EV concentrations.
