Figure 5 | Scientific Reports

Figure 5

From: Optimized Isolation of Extracellular Vesicles From Various Organic Sources Using Aqueous Two-Phase System

Figure 5

Capacity of the ATPS-based EV isolation protocol in removing non-protein contaminants. (b) BCA measurement of ascorbic acid, which cause false positives in BCA, at different steps of isolation (b) Changes in acidity after removal of ascorbic acid. (c) Removal of phenol red from culture media samples, measured via absorbance at 590 nm. (d) FAME anaylsis by gas chromatography showing the removal of lipid contaminants.

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