Figure 2
From: CETSA-based target engagement of taxanes as biomarkers for efficacy and resistance
Miniaturization of the β-tubulin CETSA assay. Schematic drawing of the principle of standard and conjugated AlphaLISA (A and B). CETSA melt curves in K562 cells treated with docetaxel or vinorelbine (20 µM). β-tubulin was detected in total and clarified cell lysate with AlphaLISA pair 2 (C and D). Cell density titration for AlphaLISA pair 2 in lysate from K562 cells treated with docetaxel (20 µM) or vehicle and heated to 37 or 63 °C (E). The data represent the mean ± S.E.M from technical replicates and are presented as a percentage of the signal detected at the lowest temperature in each melt curve.
