Figure 3 | Scientific Reports

Figure 3

From: CETSA-based target engagement of taxanes as biomarkers for efficacy and resistance

Figure 3

CETSA TE measurements correlate to sensitivity to taxanes and report on the mechanism of resistance. ITDR-CETSA for β-tubulin at 63 °C and viability assays in multidrug-resistant (K562-R) and the corresponding parental cells (K562-P) in response to increasing concentrations of docetaxel (AD) or paclitaxel (EH) and in the absence or presence of the Pgp-inhibitor tariquidar (C,D,G,H). ITDRs at 63 °C and viability assays performed in response to the non-Pgp-substrate epothilone B (I and J). β-tubulin was detected with AlphaLISA in the ITDR-CETSA experiments. Pgp- and β-tubulin-expression in the two cell lines was detected with western blot (K,L). ITDR-CETSA data are presented as relative to the compound concertation where maximum stabilization is achieved. Cell viability data is relative to the untreated samples. All data represent the mean ± S.E.M from independent experiments (n = 3–4).

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