Figure 3
From: Colorectal cancers utilize glutamine as an anaplerotic substrate of the TCA cycle in vivo
More [13C5]-glutamine enters the TCA cycle in PIK3CA mutant tumors than adjacent normal tissue in orthotopic xenograft models. [13C5]-glutamine tracing in orthotopic xenograft tumors established from WT-only (7 mice) and mutant-only (8 mice) cells. Two pieces (~1 mm3) of subcutaneous xenografts were sutured into the cecum serosa of nude mice. One day after the surgery, mice were infused with [13C5]-glutamine. The M5 enrichment of glutamine and the M4 enrichments of metabolites directly derived from M5 glutamine in WT- and mutant-only tumors are shown in (A). The M5 enrichment of glutamine and the M4 enrichments of metabolites directly derived from M5 glutamine in HCT116 mutant-only tumors and adjacent cecum tissues are shown in (B). Percentages of total 13C-labeled metabolited normalized to total 13C-labeled glutamine in the mutant-only tumors are plotted in (C). *p < 0.05, **p < 0.01, ***p < 0.001; the Student’s t test.
