Figure 5
From: Colorectal cancers utilize glutamine as an anaplerotic substrate of the TCA cycle in vivo
More [13C5]-glutamine enters the TCA cycle in PIK3CA mutant tumors than adjacent normal tissue in genetically engineered mice. CDX2P-CreERT2 Apcflox/+ KrasLSL-G12D/+ Pik3caLSL-E545K/+ mice were treated with tamoxifen to conditionally express the Kras and Pik3ca oncogenes and to delete Apc. Two moths post-treatment, mice were infused with [13C5]-glutamine. Representative image of H & E staining of a colon tumor is shown in (A). Arrow indicates a tumor invaded to the muscle layer. The M5 enrichment of glutamine and the M4 enrichments of metabolites directly derived from M5 glutamine are shown in (B). Percentages of total 13C-labeled metabolited normalized to total 13C-labeled glutamine in tumors are plotted in (C). *p < 0.05, **p < 0.01, ***p < 0.001; the Student’s t test.
