Table 1 Antibodies.
Antigen | Host, dilution | RRID | Vendor, catalog number |
|---|---|---|---|
Primary antibodies | |||
GFAP | Rabbit, 1:5000 | AB_10013382 | Agilent, Z0334 |
hCD45 | Mouse, 1:250 | AB_400305 | BD Biosciences, 347460 |
hCD3 | Rat, 1:250 | AB_321245 | Bio-Rad, MCA1477 |
hCD4 | Rabbit, 1:500 | AB_2686917 | Abcam, ab183685 |
hCD8 | Mouse, 1:250 | AB_395995 | BD Biosciences, 555631 |
HLA-DR/DP/DQ | Mouse, 1:100 | AB_794857 | Thermo Fisher Scientific, MA1-25914 |
Secondary amplification/detection | |||
Biotin-rabbit IgG | Goat, 1:1000 | AB_2313606 | Vector Labs, BA-1000 |
Biotin-rat IgG | Rabbit, 1:1000 | AB_10015300 | Vector Labs, BA-4001 |
Alexa488-rabbit IgG | Goat, 1:500 | AB_2576217 | Invitrogen, A-11034 |
Alexa546-mouse IgG | Goat, 1:500 | AB_144695 | Invitrogen, A-11030 |
Alexa633-rat IgG | Goat, 1:500 | AB_141553 | Invitrogen, A-21094 |
Flow cytometry antibodies (per test) | |||
mFc Block, CD16/32 | Rat, 1 μL | AB_394657 | BD Biosciences, 553142 |
hFc Block | Unknown, 1 μL | AB_2728082 | BD Biosciences, 564219 |
mCD45-APC | Rat, 2.5 μL | AB_398672 | BD Biosciences, 559864 |
hCD45-PerCP | Mouse, 10 μL | AB_400307 | BD Biosciences, 347464 |
hCD3-APC-H7 | Mouse, 4 μL | AB_1645731 | BD Biosciences, 641397 |
hCD3-Pe-Cy7 | Mouse, 5 μL | AB_396896 | BD Biosciences, 557851 |
hCD4-APC | Mouse, 4 μL | AB_314082 | BioLegend, 300514 |
hCD4-PerCP-Cy5.5 | Mouse, 3 μL | AB_400451 | BD Biosciences, 341653 |
hCD8-Pe-Cy7 | Mouse, 4 μL | AB_314130 | BioLegend, 301012 |
hCD19-BV510 | Mouse, 5 μL | AB_2737912 | BD Biosciences, 562947 |
hCD33-FITC | Mouse, 10 μL | AB_400047 | BD Biosciences, 340533 |
hCD69-BV421 | Mouse, 3 μL | AB_10933255 | BioLegend, 310929 |
hCXCR3-BV421 | Mouse, 3 μL | AB_2737653 | BD Biosciences, 562558 |
hIFNγ-FITC | Mouse, 3 μL | AB_394516 | BD Biosciences, 552887 |
