Figure 2
From: Unaltered intravenous prion disease pathogenesis in the temporary absence of marginal zone B cells
Temporary displacement of MZ B cells does not affect the early accumulation of PrPSc upon FDC in the spleen. Mice were treated with anti-αL and anti-α4 mAb (anti-αL + anti-α4), or isotype-matched control-Ig (n = 4 mice/group), and seven days later injected IV with ME7 scrapie prions. Spleens were analysed at 35, 70 and 105 days after IV prion exposure. (A) Immunohistohemical (IHC) analysis shows heavy prion disease-specific PrPd accumulations (upper row) were detected in association with FDC (CD21/CD35 + cells, arrows; middle row) in the B cell follicles (B220 + cells, bottom row) of mice from each treatment group. Haematoxylin (blue) was used as a nuclear counterstain. Scale bar, 100 µm. (B) Paraffin-embedded tissue immunoblotting was used to confirmed the presence of prion-specific, relatively PK-resistant, PrPSc (blue/black, arrows). Scale bar, 100 µm. (C) Similar frequencies of PrPSc + FDC were detected in the spleens of mice from each treatment group. Closed circles, control-Ig; open circles, anti-αL + anti-α4; horizontal bars, median. n = 4 mice/group.
