Table 1 Summary of cross-reactive clones by anti-HA antibody.

From: CF-PA2Vtech: a cell-free human protein array technology for antibody validation against human proteins

Clone no.

Plate no.

Well no.

Entry clone ID

Refeq ID

Protein

Short name

Similar epitope region

Similar epitope sequence*

1

1

B9/10

FLJ10511AAAF

NP_060590.1

armadillo repeat containing 1

ARMC1

233—241

QNTELPDYL

2

1

F3/4

FLJ94173AAAF

NP_004421.2

early growth response 3

EGR3

187–195

LFPMIPDYN

3

1

G19/20

FLJ40075AAAF

NP_695001.2

chromosome 20 open reading frame 96

 

18–26

QEFQVPDYV

4

1

K3/4

FLJ92933AAAF

NP_036514.1

tetratricopeptide repeat domain 33

TTC33

197–205

SPKSIPDYD

5

1

N5/6

FLJ20516AAAF

NP_060328.2

TIMELESS interacting protein

 

8–16

GVIDLPDYE

6

1

N9/10

FLJ82655AAAF

NP_981953.2

family with sequence similarity 47, member A

 

709–717

KKPDEPDVL

7

2

C15/16

FLJ10734AAAF

NP_060667.2

ZFP64 zinc finger protein

 

485–493

QPSQVPQFS

8

2

F3/4

FLJ10882AAAF

NP_071371.3

zinc finger protein 64

ZFP64

431–439

QPSQVPQFS

9

2

H5/6

FLJ81155AAAF

NP_001244031.1

NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12 kDa

NDUFB3

12–20

HKMELPDYR

10

2

K15/16

FLJ31145AAAF

NP_443089.3

zinc finger protein 830

 

152–160

GLSLLPDYE

11

3

M17/18

FLJ54287AAAF

NP_004421.2

early growth response 3, a splice variant

 

28–36

TVTMIPDYN

12

4

D11/12

FLJ82936AAAF

NP_919431.2

PH domain and leucine rich repeat protein phosphatase 1

 

1193–1201

QLDQLPDYY

13

4

F21/22

FLJ83815AAAF

NP_001955.1

early growth response 1

EGR1

248–256

QVPMIPDYL

HA

       

YPYDVPDYA

  1. *The amino acids identical with HA epitope sequence are shown as bold.