Figure 1
From: The Adult Murine Intestine is Dependent on Constitutive Laminin-γ1 Synthesis
Conditional deletion of the Lamc1 gene in adult mice rapidly leads to laminin protein depletion in the small intestine. For (A,B), tissues include heart (Ht), Lung (Lu), Kidney (Ki), Liver (Lv), Spleen (Sp) and Small Intestine (In). (A) Tissue from tamoxifen-treated control (CT), tamoxifen-treated mutant (MT), and untreated mutant (MN) were analyzed by PCR for the presence of wild type Lamc1, Lamc1LoxP, and recombined Lamc1LoxP−. The lane marked * contains tail DNA from a mouse with the genotype Lamc1LoxP/+. (B) Tissue lysates were analyzed by ELISA for total laminin protein concentration. Each data point is the average of two technical replicates from a single animal. (C) Immunofluorescent images of control and mutant small intestine that were probed with antibody against laminin-γ1 (yellow). Endogenous mTomato and eGFP are also shown. DAPI was used to label nuclei (blue). Magnification bars indicate 50 microns (lower magnification) and 10 microns (higher magnification). 10x and 40x with a 1.5 magnifier lenses were used to collect these images. Images are representative of the results obtained from immunofluorescent analysis of 3 tamoxifen-treated control and 4 tamoxifen-treated mutant mice.
