Figure 2

Binding and activation of MOR. (A) Displacement of bound [³H]-DAMGO (4 nM) by FF6 at pH 6.5 and 7.4. (B) IC₅₀ calculated from (A). P > 0.05, unpaired t-test (n = 6–7). (C) [³⁵S]-GTPγS binding induced by FF6 at pH 6.5 and 7.4. [³⁵S]-GTPγS binding is expressed as percent increase in [³⁵S]-GTPγS binding relative to binding in unstimulated samples (n = 6). (D) EC₅₀ of FF6 from (C). P > 0.05, unpaired t-test. Data are presented as mean ± SEM (A, C) and as mean ± 95% confidence intervals (B, D) (n = 6).