Figure 3

Adherent S-cNS-derived cells, but not GF-derived cells, differentiate into GCs in appropriate conditions. Immunostaining for β3-TUBULIN (green), GFAP (green), VGLUT1 (red) and GABRA6 (red) proteins in S-cNS and GF-cNS -derived cells grown onto polylysine-coated substrates, and treated with differentiation medium containing FBS + VitA, for 96 hours. The images are representative of three independent experiments. Cell nuclei are evidenced (in blue) by HOECHST- staining. Scale bar, 10 μm.