Figure 5
Cerebellar explants from Ptch1-KO, but not SmoA1, mice allow generation of neurospheres without SAG stimulation. (A) Neurosphere formation assay on P7 WT, SmoA1 and Ptch1-KO cerebellar explants with or without SAG. Data obtained by three independent experiments are reported as means +/− SD. (*p < 0.05; ***p < 0.001). (B) Neurosphere formation assay on P7 WT and SmoA1 cerebellar explants, with different SAG concentrations. Data obtained by three independent experiments are reported as means +/− SD. (*p < 0.05; ***p < 0.001). (C) WB analysis of the indicated proteins in S-cNS cultures derived from the same explants and then treated as in (B). (D) WB analysis of GLI1 expression in WT and SmoA1 S-cNS exposed to SAG, or SAG-deprivation (SAG−) and collected at the indicated time points. Blots were probed for β-actin as a loading control. Uncropped Western blot images related to this figure are displayed in Suppl. Fig. 11.
