Figure 7
Different features of SmoA1 and Ptch1-KO mouse models during postnatal cerebellar development. (A) WB analysis of the indicated proteins in total extracts from WT, SmoA1 and Ptch1-KO cerebella, collected at different stages of post-natal development. Ponceau staining was used as a loading control. (B) Sagittal sections from P14 and P21 WT, SmoA1 and Ptch1-KO mouse cerebella were analyzed for Ki67 expression by immunohistochemistry (magnification 20X). The inset shows in greater detail an area containing a high concentration of resting cells, which are negative for Ki67 immunoreactivity, in a representative region of P21 SmoA1 cerebella. EGL: External Granular Layer; ML: Molecular Layer; IGL: Internal Granular layer. Uncropped Western blot images related to this figure are displayed in Suppl. Fig. 13.
