Figure 4
Representative chromatogram of [Pyr1]apelin-13 and its C-terminal metabolites identified in human plasma. The extracted ion chromatogram for [Pyr1]apelin-13 shows the m/z for the first 13C ion, as the extracted chromatogram for the monoisotopic peak had significant background noise throughout. (A) [Pyr1]apelin-13 with 8.80 minutes retention time; (B) [Pyr1]apelin-13(1–12) with 6.26 minutes retention time; (C) [Pyr1]apelin-13(1–10) with 4.29 minutes retention time; (D) [Pyr1]apelin-13(1–8) with 3.25 minutes retention time; (E) [Pyr1]apelin-13(1–7) with 4.22 minutes retention time; (F) [Pyr1]apelin-13(1–6) with 5.09 retention time; (G) [Pyr1]apelin-13(1–5) with 5.88 minutes retention time. These data were acquired using Orbitrap Mass spectrometer used for metabolite identification. The mass accuracy of the experimentally acquired monoisotopic peak was calculated for each potential metabolite, and is included along with the 13C isotopic cluster for each peptide with their corresponding chromatogram.
