Table 1 Validation of linearity, sensitivity, and reproducibility of the ELISA with egg derived HAs.
Egg-derived HA (NIBSC) | uAb (group 2 IAV) | uAb (IBV) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
4F11 | 10F8 | |||||||||
Type | HA subtype | Strain | Response | Linearity (R2) | Sensitivity (LOD (µg/ml)) | Reproducibility (%CV) | Response | Linearity (R2) | Sensitivity (LOD (µg/ml)) | Reproducibility (%CV) |
Group 1 IAV | H1 | A/California/7/09 (NYMC-X181) | — |  |  |  | — |  |  |  |
A/Brisbane/59/2007 (IVR-148) | — |  |  |  | — |  |  |  | ||
H2 | A/Singapore/1/57 | — |  |  |  | — |  |  |  | |
H5 | A/Anhui/1/05 IBCDC-RG-6 | — |  |  |  | — |  |  |  | |
Group 2 IAV | H3 | A/Hong Kong/4801/2014 (NYMC X-263B) | + | 0.995 | 0.011 | 4.913 | — |  |  |  |
A/Switzerland/9715293/2013 (NIB88) | + | 0.996 | 0.004 | 4.191 | — |  |  |  | ||
A/Texas/50/2012 (NYMC X-223A) | + | 0.996 | 0.010 | 5.800 | — |  |  |  | ||
H7 | A/mallard/Netherlands/12/2000 | + | 0.999 | 0.011 | 4.856 | N/A | Â | Â | Â | |
A/Anhui/1/2013 | + | 0.999 | 0.017 | 5.610 | N/A | Â | Â | Â | ||
IBV | Yamagata | B/Phuket/3073/2013 | - | Â | Â | Â | + | 1.000 | 0.004 | 3.799 |
B/Massachusetts/02/2012 | — |  |  |  | + | 0.993 | 0.006 | 6.250 | ||
Victoria | B/Brisbane/60/2008 (NYMC BX-35) | — |  |  |  | + | 0.980 | 0.000* | 5.287 | |
B/Maryland/15/2016 | N/A | Â | Â | Â | + | 0.994 | 0.002 | 6.098 | ||
