Figure 5
Quantitative PCR analysis of selected genes. The results of two experiments are presented. Experiment 1 used the same methodology as the RNA sequencing analysis to generate samples. Experiment 2 used the methodology of Seifbarghi et al. (2017)13. The y axis shows the inverse ∆Ct value relative to the housekeeping gene Sclerotinia sclerotiorum β-tubulin. The x axis shows the names of genes included in the analysis. The gene names coloured in green correspond with the RNA sequencing analysis, whereas those in red do not. The thick horizontal black lines represent median values. The boxes and whiskers represent interquartile range and the black points represent outliers. For most of the genes, we were concerned with differential expression between Lupinus angustifolius and Brassica napus, as this was the primary hypothesis we were testing. We also selected the two genes sscle_15g106480 and sscle_15g106510 as they were significantly up-regulated on both B. napus and L. angustifolius relative to in vitro.
