Figure 2
From: Crebl2 regulates cell metabolism in muscle and liver cells
Crebl2 knockdown increases fat content in mouse myoblasts and hepatoma cells. (A) Knockdown of Crebl2 increases triglyceride (TAG) content of C2C12 myoblasts. C2C12 were treated with control or two independent siRNAs against Crebl2 for 3 days before TAG measurement, normalized to total cell protein. (n = 3). (B) Knockdown of Crebl2 increases triglyceride content of Hepa1-6 hepatoma cells. Hepa1-6 cells were treated with control or two independent siRNAs against Crebl2 for 3 days before fat measurement, normalize to total cell protein. (n = 3). (C,C’) Knockdown of Crebl2 does not affect lipid uptake of C2C12 or Hepa1-6 hepatoma cells. Hepa1-6 cells were treated with control or siRNAs against Crebl2 for 3 days before lipid uptake assay. (n = 3). (D) Knockdown of Crebl2 increases glucose uptake in C2C12 myoblasts. C2C12 myoblasts were treated with control or two independent siRNAs against Crebl2 for 3 days before glucose uptake assay, normalized to total cell protein. (n = 3). (E) Knockdown of Crebl2 does not affect glucose uptake of Hepa1-6 cells. Hepa1-6 cells were treated with control or two independent siRNAs against Crebl2 for 3 days before glucose uptake assay, normalized to total cell protein. (n = 3). Error bars: SD ***t-test < 0.001, **t-test < 0.01, *t-test < 0.05.
