Table 1 Primer sequences specific to target genes.

From: Differential IL-17A response to S. pneumoniae in adenoid tissue of children with sleep disordered breathing and otitis media with effusion

 

Forward primers

Reverse primers

IL-12A

TTCACCACTCCCAAAACCTG

AATGGTAAACAGGCCTCCAC

IFN-Îł

GCC AAC CTA AGC AAG ATC CCA

ATT TGG AAG CAC CAG GCA TGA

IL-4

TTTGCTGCCTCCAAGAACACA

TCCTGTCGAGCCGTTTCAG

IL-5

AGACCTTGGCACTGCTTTCT

CAGTACCCCCTTGCACAGTT

IL-17A

TTGGTGTCACTGCTACTGCT

TTGGGCATCCTGGATTTCGT

IL-22

AGCCCTATATCACCAACCGC

TCTCCCCAATGAGACGAACG

IL-10

CTCTGATACCTCAACCCCCAT TT

GAGGGAGGTCAGGGAAAACAG

TGF-β

CTG CGG ATC TCT GTG TCA TT

TGC CCA AGG TGC TCA ATA AA

RORÎłt

CCCAGAACCTCTCTTGGCTT

GAATGGCACAAGTTGGGGTT

FOXP3

AGAGAGCCTGCCTCAGTACA

TGACGCTGCTTCTGTGTAGG

GADPH

TTCCAGGAGCGAGATCCCT

CACCCATGACGAACATGGG

  1. IFN-γ, Interferon gamma; TGF-β, Transforming growth factor beta; RORγt, RAR-related orphan receptor gamma t; FOXP3, Forkhead box P3; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
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