Figure 1
From: AAV-mediated expression of HLA-G1/5 reduces severity of experimental autoimmune uveitis
AAV8 gene delivery to EAU Iris and Ciliary Body. Representative histological images of GFP immunofluorescence following intravitreal injection of AAV8-GFP. Transgene expression (green) was examined by immunostaining. The left image is of the iris and ciliary body, without primary antibody; the right image is with anti-GFP antibody. To confirm gene delivery to inflamed uvea, rats were injected with either scAAV8-GFP (3 × 1010 viral genomes; 3 μL; n = 6) or balanced salt solution (3 μL; n = 3) and experimental autoimmune uveitis was induced in 3 of the AAV-GFP injected rats and 3 balanced salt solution rats one week following intraocular injections. Animals were euthanized 2 weeks following induction of EAU and tissues were collected for histopathology. The results demonstrate strong GFP staining in cells of inflamed ciliary body and iris following intravitreal injection of AAV8-GFP. The control section (left), without primary antibody, shows several focal areas of green fluorescence, assumed to be autofluorescence of red blood cells based on the anatomic location of the fluorescence.
