Figure 2

ABC transporter gene knockdown modulates PPN hatching, attraction and invasion. Hatching of J2s in response to experimental treatment (root exudates or water) as a factor of time, for (A) M. incognita; (B) M. javanica; and (C) G. pallida. Error bars represent SEM. (D) Chemosensory challenge of Meloidogyne and Globodera spp. A positive chemosensory index (CI) equates to attraction towards tested exudates, and a negative CI infers repulsion from the exudates. Data represent five biological replicates for each nematode species in each treatment group. (E) The number of galls formed (Meloidogyne spp.) or developing females found on the root surface (G. pallida) six weeks post inoculation on VIGS plants (n = 10 plants per species). Asterisks indicate statistical significance relative to controls following one-way ANOVA and Tukey’s HSD tests: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.