Figure 5
From: Effect of nicotine on Staphylococcus aureus biofilm formation and virulence factors
Nicotine enhanced the release of extracellular DNA (eDNA). The amount of eDNA in the matrix of S. aureus biofilm(24 h) were determined by measuring the fluorescence of PI-bound eDNA with the excitation/emission wavelength at 535/610 nm. Relative amount of eDNA was expressed as the fluorescence intensity per OD600 unit. The amount of eDNA was higher in the nicotine treatment group (15.95 ± 1.64, n = 3) as compared to the control group (10.59 ± 0.51, n = 3) *P < 0.05.
