Figure 2

Vascular repair in the peri-infarct region following stroke in response to VEGF and anti-Nogo-A antibodies. (A) Schematic representation of the analyzed cortical region and experimental timeline in the four experimental groups: Ctrl-Ab (N = 6), VEGF (N = 7), α-Ng-Ab (N = 5), Combined (VEGF and α-Ng-Ab) (N = 7). (B) Overview of the assessed vascular parameters including the vascular area fraction (area), number of branch points (branches), vessel segment length (length) and nearest neighbor distance and variability (distance). (C) Fluorescent images of blood vessels (CD31⁺) in the peri-infarction region in the four treatment groups. Scale bar = 100 μm. (D) Quantitative evaluation of the vascular area fraction, number of branches, vessel segment length, nearest neighbor distance and variability in the peri-infarct region. (E) Experimental timeline of EdU injections. (F) Fluorescent images of newly formed blood vessels (EdU⁺ - pink; CD31⁺ - white), Scale bar = 50 μm. (G) Quantification of EdU⁺/CD31⁺ cells in the peri-infarct region. Data are represented as mean ± SD. Each dot in the plots represents one animal and significance of mean differences between the groups was assessed using Tukey’s HSD. Asterisks indicate significance: *P < 0.05, **P < 0.01, ***P < 0.001. CTX, cortex; CC corpus callosum.