Figure 4 | Scientific Reports

Figure 4

From: Specific strains of Streptococcus mutans, a pathogen of dental caries, in the tonsils, are associated with IgA nephropathy

Figure 4

Double IF staining for Cnm and CD68 on tonsillar sections from IgAN patients. (A–D) Double IF staining for Cnm (Alexa Fluor 488: green) and CD68 (Alexa Fluor 594: red) and counterstaining of nuclei (DAPI: blue) were performed using tonsillar sections previously stained with immunoperoxidase for Cnm. (A) Cnm was predominantly observed in the germinal centers (GC) or surrounding area, with the surrounding area being more dominant. (B) CD68, a macrophage marker, was observed in the germinal centers and surrounding area. (C) Nuclear staining with DAPI. (D) Merged image of (A–C). Arrows indicate Cnm-positive macrophages (original magnification, A-D, × 20). Findings suggest that Cnm coexist with macrophages in the tonsils.

Back to article page